Illumina Sample Preparation info


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Library Preparation

  Truseq Nano DNA Guidetruseq-nano-dna-lt-sm

The TruSeq Nano DNA HT Sample Prep Kitprovides a simple, scalable, cost-effective solution for generating libraries from genomic DNA that is compatible with Illumina’s unparalleled sequencing output. The kit features a 96-well plate with 96 uniquely indexed adapter combinations designed for manual or automated preparation of 96 uniquely indexed samples.

Catalog IDs: FC-121-4003

The TruSeq Nano DNA LT Sample Prep Kit provides a simple, scalable, cost-effective solution for generating libraries from genomic DNA that is compatible with Illumina’s unparalleled sequencing output. The kits feature 24 unique indexes, delivering enhanced multiplex performance for processing up to 384 samples on a single HiSeq 2000 run.

Catalog IDs: FC-121-4001, FC-121-4002

TruSeq Nano DNA prep - Full Service
InternalExternal non-profitExternal for-profit
$108$148Please contact for a quote

 

 

Truseq Stranded mRNA Guide truseq_rna_stranded_lt_sm

The TruSeq Stranded mRNA LT Sample Prep Kit provides a simple, cost-effective solution for generating libraries from total RNA that are compatible with Illumina’s unparalleled sequencing output. The kits feature 24 unique indexes, delivering enhanced multiplex performance for processing up to 384 samples on a single HiSeq 2000 run.

Catalog IDs: RS-122-2101, RS-122-2102

The TruSeq Stranded mRNA HT Sample Prep Kit provides a simple, cost-effective solution for generating libraries from total RNA that are compatible with Illumina’s unparalleled sequencing output. The kits feature 24 unique indexes, delivering enhanced multiplex performance for processing up to 384 samples on a single HiSeq 2000 run.

Catalog IDs: RS-122-2103

TruSeq Stranded mRNA prep - Full Service
InternalExternal non-profitExternal for-profit
$160$200Please contact for a quote
 

 Nextera XT Guide (1ng starting material) nextera_xt_sample_96-2

Nextera XT DNA Sample Preparation kits provide the fastest and easiest workflow, enabling sequencing-ready libraries to be generated in less than 90 minutes, with less than 15 minutes of hands-on time. DNA is simultaneously fragmented and tagged with sequencing adapters in a single step, using standard lab equipment. Libraries prepared with Nextera XT kits are compatible with all Illumina sequencers.

 Catalog IDs: FC-131-1024, FC-131-1096, FC-131-1001, FC-131-1002, FC-121-1003, PE-121-1003

Nextera XT prep - Full Service
InternalExternal non-profitExternal for-profit
$74$114Please contact for a quote
 

 

 

 Nextera Guide (50ng starting material) nextera_dna_sample_prep_kit_th

Nextera DNA Sample Preparation kitsprovide the fastest and easiest workflow, enabling sequencing-ready libraries to be generated in less than 90 minutes, with less than 15 minutes of hands-on time. DNA is simultaneously fragmented and tagged with sequencing adapters in a single step, using standard lab equipment. Libraries prepared with Nextera kits are compatible with all Illumina sequencers.

 Catalog IDs: FC-121-1031, FC-121-1030, FC-121-1012, FC-121-1011, FC-121-1003, PE-121-1003


 

 

 

 

 Clontech SMARTer Guide  (for use with Nextera)  Logo-Clontech

SMARTer Ultra Low Input RNA Kit for Illumina Sequencing—cDNA Synthesis from Single Cells 

The SMARTer Ultra Low RNA Kit for Illumina® Sequencing provides a simple and efficient solution for generating libraries for transcriptome analysis (mRNA-seq) from single cells, a few to 1,000 intact cells, or very low amounts of total RNA (10 pg–10 ng) that are compatible with Illumina’s Genome Analyzer™, HiSeq®, or MiSeq® instruments. This kit uses oligo dT priming and allows cDNA synthesis directly from cells without any sample preparation or can use total RNA as the starting material. Clontech offers multiple kits for single cell transcriptome analysis. This kit requires an input volume of 1 µl. The SMARTer Ultra Low Input RNA for Illumina Sequencing – HV accommodates higher input volumes of 1–9 µl of cells or RNA. 

 

The SMARTer Ultra Low RNA Kit for Illumina has also been reconfigured specifically for the single-cell mRNA-seq application on the Fluidigm C1 System. The core SMART technology in these kits is referred to as SMART-seq™ in the literature, and has shown to be an immensely powerful tool for studying full gene coverage, transcript counting, splice junctions and alternative splice variants in cells.

 

 Kapa qPCR Libary Quantification Guide2013UCalgaryLogoKAPABiosystems

We recommend using Kapa qPCR kits for sensitive quantification of your final libraries.    

Catalog IDs: kk4824  (Universal kit)